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Global Journal of Parasitology

Global Journal of Parasitology - Volume 1

NETosis Induced by Trypanosoma cruzi Parasite, and Its Soluble Antigens, Occurs in the Absence of Classic Apoptosis and Necrosis Signs

Published on: 31 December 2015

Pages: 29-37

Priscila Silva Sampaio de Souza1, Larissa FigueiredoAlves Diniz1, Daniel Sousa-Rocha1, Mariana Thomaz-Tobias1, Phileno Pinge-Filho2 and Karina Alves Toledo1

1Department of Biological Sciences, Univ. EstadualPaulista – UNESP (FCL), Assis, São Paulo, Brazil and 2Department of Pathological Sciences, Center of Biological Sciences, Univ. Estadual de Londrina, Londrina, Paraná, Brazil

Abstract:

Diseases caused by protozoa is a serious public health problem displaying high mortality rate. Chagas Disease, which etiological agent is the Trypanosomacruzi, may present fatal manifestations. The controlling of the replication of this parasite involves intense and persistent innate immunological response. The neutrophils display classical microbicidal mechanisms and release DNA fibers, or NETs (Neutrophil Extracellular Traps) in response to an infectious stimulus. The NETs capture and kill various pathogens, contributing to the elimination of these by the host. Recently it was demonstrated that T. cruziand its soluble antigens stimulate the formation and release of NETs in human neutrophils. The process, denominated NETosis, is still the subject of several studies, being characterized by displaying distinct morphological and molecular signals from those presented by apoptosis and necrosis processes. This study investigated the signs of apoptosis and necrosis in human neutrophils under T. cruzistimulus and its soluble antigens. The results obtained by flow cytometry show that incubated neutrophils with T. cruzi parasite and its soluble antigens do not display any signs of DNA degradation, phosphatidylserine exposure nor loss of cell permeability. Besides that, MTT and neutral red assays indicate maintenance of cell viability during NETosis process induced by the parasite and its soluble antigens. The overall results reaffirm NETosis as a distinct process of apoptosis and necrosis and may assist in the development of new strategies for controlling the inflammatory responses during infection by T. cruzi aiming to control the parasitic load and the associated pathogenesis.

Keywords: NETose, Neutrophil Extracellular Traps, Trypanosomacruzi, Apoptosis, Necrosis.

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