Global Journal of Parasitology

Global Journal of Parasitology - Volume 1

Contribution of 5-Lipoxygenase on Macrophage Activation of Mice Acutely Infected with Trypanosomacruzi

Published on: 15 December 2015

Pages: 16-21

Daniel SanzioGimenes da Cruz1, NagelaGhabdan Zanluqui1, AparecidaDonizette Malvezi1, Vera Lúcia HidekoTatakihara1, WaldiceuAparecidoVerri, Jr.1, Lucy Megumi Yamauchi2, Sueli Fumie Yamada-Ogatta2 and Phileno Pinge-Filho1

1Departamento de CiênciasPatológicas, UniversidadeEstadual de Londrina, Londrina, Paraná, Brasil and 2Departamento de Microbiologia, UniversidadeEstadual de Londrina, Londrina, Paraná, Brasil


Chagas disease or American trypanosomiasis is a zoonosis caused by the hemoflagellate protozoan Trypanosomacruzi. In the present work, the contribution of 5-lipoxygenase (5-LO) on the spontaneous production of hydrogen peroxide (H2O2), superoxide (O2-) and nitric oxide (NO) by peritoneal macrophages from acutely T. cruzi-infected mice was examined. 5-LO gene knockout (5-LO-/-) mice and wild-type (WT) mice were infected with 5 x 103T. cruzitrypomastigote forms (Y strain) intraperitoneally. At the seventh or fourteenth day, peritoneal cells were harvested and seeded in 96 wells tissue culture plates. Supernatants of macrophage cultures were collected for NO quantification and adherent cells were used for O2- and H2O2 determination. Therefore, we contribute to understanding the involvement of leukotrienes (LTs) on the production of oxygen and nitrogen intermediates in acute T. cruzi infection.

Keywords: Chagas disease, 5-LO, H2O2, O2-, Nitric oxide.

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